SC35 is an RRM-containing SR protein that plays a
role in alternative splicing. It was shown recently that SC35 can
autoregulate its own expression. This is accomplished via a feedback
loop that connects the level of SC35 protein to the productive splicing
of the SC35 transcript.
SC35 is an RRM-containing SR protein that plays a role in alternative splicing. It was shown recently that SC35 can autoregulate its own expression. This is accomplished via a feedback loop that connects the level of SC35 protein to the productive splicing of the SC35 transcript. |
Increased concentrations of SC35 protein change the splice pattern of the SC35 transcript. The SC35 protein binds to its own pre-mRNA, uncovering splice sites downstream of the termination codon. All isoforms of SC35 share the same open reading frame and, therefore, encode the same protein. However, when these alternative splice sites in the 3' untranslated region are used, the normal termination codon becomes a premature termination codon based on the 50 nucleotide rule. NMD then degrades the transcript, down-regulating the expression of SC35. In this way, SC35 appears to auto-regulate its own production, by RUST. |
Sureau
A, Gattoni R, Dooghe Y, Stevenin J, Soret J.
SC35 autoregulates its expression by promoting splicing
events that destabilize its mRNAs.
EMBO J. 2001 Apr 2;20(7):1785-96.
Fu
XD, Maniatis T.
Isolation of a complementary DNA that encodes the
mammalian splicing factor SC35.
Science. 1992 Apr 24;256(5056):535-8.